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KMID : 1059519930370121035
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Journal of the Korean Chemical Society
1993 Volume.37 No. 12 p.1035 ~ p.1046
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Separation and Elution Behavior of Some Iron(Ⅲ)porphyrin Complexes by Reversed-Phase Liquid Chromatography
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Kang Chang-Hee
Kim In-Whan
Lee Won
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Abstract
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Some iron(Ⅲ)porphyrin complexes were prepared, and identified by the spectroscopic methods. Elution behavior of iron(Ⅲ)porphyrin complexes was investigated by reversed-phase HPLC. The optimum conditions for the separation of iron(Ⅲ)porphyrin complexes were examined with respect to flow rate and mobile phase strength. These complexes were successfully separated on NOVA-PAK C18 column using methanol / water(95/5) for [TpCF3PP)Fe(R)] and methanol / water (98/2) for [(P)Fe(C6F5)] as a mobile phase. It was found that these complexes were largely eluted in an acceptable range of capacity factor value (0≤logk'≤1). The dependence of the capacity factor (k') on the volume fraction of water in the binary mobile phase as well as the dependence of k' on the liquid-liquid extraction distribution ratio (Dc) in methanol-water / n-pentadecane extraction system showed a good linearity. It means that the retention of iron(Ⅲ)porphyrin complexes on NOVA-PAK C18 column is largely due to the solvophobic effect. Also, there was a good linear dependence of the capacity factor(k') on the column temperature and enthalpy calculated by van't Hoff plot. From these results, it was confirmed that the retention mechanism of iron(Ⅲ)porphyrin complexes in reversed-phase liquid chromatography was invariant under the condition of various temperature, and the solvophobic binding process exhibited isoequilibrium behavior.
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